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pcdna-gfp-stat1 plasmid #11987  (Addgene inc)


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    Structured Review

    Addgene inc pcdna-gfp-stat1 plasmid #11987
    Pcdna Gfp Stat1 Plasmid #11987, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat1+gfp/pegfp+c1+stat1/pmc11986988-49-4-6
    Average 90 stars, based on 1 article reviews
    pcdna-gfp-stat1 plasmid #11987 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: SARS-CoV-2 Orf6 hijacks Nup98 to block STAT nuclear import and antagonize interferon signaling
    Article Snippet: .. The expression vectors for HANS5 ZKV (Uganda strain)(1), STAT1-GFP, STAT2-RFP(2), Myc-NUP98 (3), pRL-TK, and ISG54Firefly luciferase plasmid have been previously described (4). pCMVTNT-T7-KPNA1 and pCMVTNT-T7-KPNA2 were a gift from Bryce Paschal (Addgene #26677, and #26678)(5). pEGFPN1 Full length Importin beta was a gift from Patrizia Lavia (Addgene plasmid #106941)(6). .. All HEK293T and Vero E6 transfections were performed using TransIT-LT1 (Mirus Bio) according to the manufacturer’s instructions.

    Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling
    Article Snippet: ON-TARGETplus siRNA (Dharmacon no. L-003543-00-0005) and Risc-free control siRNA (Dharmacon no. D-001600-01-05) were used for silencing STAT1 expression . .. Expression vectors for JAK2-HA, DN-JAK2-HA (gifts of I. Daar, NCI, NIH), STAT1-GFP (Addgene, no 11987) and JNK3 mCherry (GeneCopoeia, EX-A1150-M55) were transfected into HUVEC and HDMEC . .. MISSION® shRNA was used for silencing JNK3 expression (Sigma-Aldrich, no. SHCLND-NM_002753).

    Luciferase:

    Article Title: SARS-CoV-2 Orf6 hijacks Nup98 to block STAT nuclear import and antagonize interferon signaling
    Article Snippet: .. The expression vectors for HANS5 ZKV (Uganda strain)(1), STAT1-GFP, STAT2-RFP(2), Myc-NUP98 (3), pRL-TK, and ISG54Firefly luciferase plasmid have been previously described (4). pCMVTNT-T7-KPNA1 and pCMVTNT-T7-KPNA2 were a gift from Bryce Paschal (Addgene #26677, and #26678)(5). pEGFPN1 Full length Importin beta was a gift from Patrizia Lavia (Addgene plasmid #106941)(6). .. All HEK293T and Vero E6 transfections were performed using TransIT-LT1 (Mirus Bio) according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: SARS-CoV-2 Orf6 hijacks Nup98 to block STAT nuclear import and antagonize interferon signaling
    Article Snippet: .. The expression vectors for HANS5 ZKV (Uganda strain)(1), STAT1-GFP, STAT2-RFP(2), Myc-NUP98 (3), pRL-TK, and ISG54Firefly luciferase plasmid have been previously described (4). pCMVTNT-T7-KPNA1 and pCMVTNT-T7-KPNA2 were a gift from Bryce Paschal (Addgene #26677, and #26678)(5). pEGFPN1 Full length Importin beta was a gift from Patrizia Lavia (Addgene plasmid #106941)(6). .. All HEK293T and Vero E6 transfections were performed using TransIT-LT1 (Mirus Bio) according to the manufacturer’s instructions.

    Transfection:

    Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling
    Article Snippet: ON-TARGETplus siRNA (Dharmacon no. L-003543-00-0005) and Risc-free control siRNA (Dharmacon no. D-001600-01-05) were used for silencing STAT1 expression . .. Expression vectors for JAK2-HA, DN-JAK2-HA (gifts of I. Daar, NCI, NIH), STAT1-GFP (Addgene, no 11987) and JNK3 mCherry (GeneCopoeia, EX-A1150-M55) were transfected into HUVEC and HDMEC . .. MISSION® shRNA was used for silencing JNK3 expression (Sigma-Aldrich, no. SHCLND-NM_002753).



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    Image Search Results


    Journal: iScience

    Article Title: Transcriptional synergy in human aortic endothelial cells is vulnerable to combination p300/CBP and BET bromodomain inhibition

    doi: 10.1016/j.isci.2024.110011

    Figure Lengend Snippet:

    Article Snippet: 3xFLAG-STAT1 Plasmid , Addgene , 220323.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Virus, Sample Prep, SYBR Green Assay, CRISPR, Negative Control, Software, Plasmid Preparation